高通量甲基化研究技术-芯片和二代测序

高通量甲基化研究技术-芯片和二代测序

Schematic outline of MeDIP. Genomic DNA is sheared into ~400-700bp using sonication and subsequently denatured. Incubation in 5mC antibodies, along with standard immunoprecipitation, enriches for fragments that are methylated (IP fraction). This IP fraction can become the input sample to one of two DNA detection methods: array hybridization using high-density microarrays (A) or high-throughput sequencing using the latest in sequencing technology (B). Output from these methods are then analyzed for methylation patterns to answer the biological question.

 

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上海欧易生物医学科技有限公司成立于2010年初,是在上海欧易生物科技有限公司(成立于2004年)的基础上快速发展起来的专业技术服务公司,公司服务于生命科学领域,为临床与基础研究客户提供分子生物学高端技术服务。

欧易生物汇聚了生物技术服务领域的一批优秀人才,核心管理团队平均十年以上的行业从业经验,经过两年的发展,已经在高通量测序技术、生物芯片技术、文库构建技术、实时定量 PCR 技术、 SNP分型技术、microRNA检测技术、甲基化检测技术,蛋白质研究技术等方向建立起完善的服务体系,并服务了大量客户。另外,欧易技术平台还在紧张地进行新的技术的开发及引进工作,力求将当下最先进的技术服务带给国内研究领域客户。

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